Application procedures list

This information relates to Question B9 on the AEC application form.

Researchers are requested to bold the most appropriate section in the table in question B9 of the AEC application form.  This purpose should reflect the primary procedure of the protocol only.

Procedure Number

Description

P1

Observation Involving Minor Interference

Animals are not interacted with or, where there is interaction, it would not be expected to compromise the animal’s welfare any more than normal handling, feeding, etc.  There is no pain or suffering involved.

Examples

  • Observational study only
  • Breeding animals for supply, where only normal husbandry procedures are used
  • Breeding or reproductive study with no detriment to the animal
  • Feeding trial, such as Digestible Energy determination of feed in a balanced diet
  • Behavioural study with minor environmental manipulation
  • Teaching or normal, non-invasive husbandry such as handling, grooming, etc

P2

Animal Unconscious Without Recovery

Animal is rendered unconscious under controlled circumstances with little or no pain or distress.  Capture methods are not required.  Any pain is minor or brief and does not require analgesia.  Procedures are carried out on the unconscious animal which is then killed without regaining consciousness.

Examples

  • Laboratory animals killed painlessly for dissection, biochemical analysis, etc
  • Teaching surgical techniques on live, anaethetised patients which are not allowed to recover following the procedure

P3

Minor Conscious Intervention

Animal is subjected to minor procedures which would normally not require anaesthesia or analgesia.  Any pain is minor and analgesia usually unnecessary, although some distress may occur as a result of trapping or handling.

Examples

  • Injections, blood sampling in conscious animal
  • Minor dietary or environmental deprivation or manipulation, such as feeding nutrient-deficient diets for short periods
  • Trapping and release as used in species impact studies, etc
  • Trapping and humane euthanasia for collection of specimens
  • Stomach tubing, shearing

P4

Minor Surgery With Recovery

Animal is rendered unconscious with as little pain or distress as possible.  A minor procedure such as cannulation or skin biopsy is carried out and the animal is allowed to recover.  Depending on the procedure, pain may be minor or moderate and post-operative analgesia may be appropriate. Field capture using chemical restraint methods is also included here.

Examples

  • Biopsies
  • Cannulations
  • Sedation/anaesthesia for relocation, examination or injections/blood sampling

P5

Major Surgery With Recovery

Animal is rendered unconscious with as little pain or distress as possible.  A major procedure such as abdominal or orthopaedic surgery is carried out and the animal is allowed to recover.  Post operative pain is usually considerable and at a level requiring analgesia.

Examples

  • Orthopaedic surgery
  • Abdominal or thoracic surgery
  • Transplant surgery
  • Mulesing, castration without anaesthesia

P6

Minor Physiological Challenge

Animal remains conscious for some or all of the procedure.  There is interference with the animal’s physiological or psychological processes.  The challenge may cause only a small degree or pain/distress or any pain/distress is quickly and effectively alleviated.

Examples

  • Minor infection
  • Minor or moderate phenotypic modification
  • Early oncogenesis
  • Arthritis studies with pain alleviation
  • Induction of metabolic disease
  • Prolonged deficient diets
  • Polyclonal antibody production
  • Antiserum production

P7

Major Physiological Challenge

Animal remains conscious for some or all of the procedure.  There is interference with the animal’s physiological or psychological processes.  The challenge may cause only a moderate or large degree or pain/distress which is not effectively alleviated.

Examples

  • Major infection
  • Major phenotypic modification
  • Oncogenesis without pain alleviation
  • Arthritis studies with no pain alleviation
  • Uncontrolled metabolic disease
  • Isolation or environmental deprivation for extended periods
  • Monoclonal antibody raising in mice

P8

Death As An Endpoint

This category only applies in those rare cases where the death of an animal is a planned part of the procedures and animals die but are not euthanased.  Where predictive signs of death have been determined and euthanasia is carried out before significant suffering occurs, they may be placed in category 6 or 7.

Examples

  • Lethality testing (including LD50, LC50)

It does not include: death by natural causes; animals which are euthanased as part of the project; animals which are euthanased if something goes wrong; animals euthanased for dissection or for use as museum specimens; or accidental deaths.

P9

Production Of Genetically Modified Animals

This category is intended to allow for the variety of procedures which occur during the production of genetically modified animals.  As animals in this category may be subjected to both minor and major physiological challenges and surgical procedures, this category reflects the varied nature of the procedures carried out.  It effectively includes ALL animals use in GM productions other than the final progeny which are used in a different category of procedure.

Examples

  • Initial breeding animals for GM production
  • Animals culled as part of the GM production process