PIIC Molecular and Microarray Laboratory
A molecular and microarray laboratory facility has been established at UNE under the Primary Industries Innovation Centre (PIIC). The laboratory facility is available to the UNE, NSW DPI and partners of TIGB research community.
Laboratory capability
General molecular biology approaches:
- DNA/RNA extractions
- PCR (high throughput)
- Robotic liquid handling
- Agarose/ polyacrylamide gel electrophoresis
- DNA cloning
- DNA sequencing
Molecular marker system:
- Microsatellite analysis
- SNPs (High Resolution Melt curve analysis or HRM)
- RAPD
Gene mapping:
- Linkage mapping (Joinmap etc)
- QTL mapping (QTL Express etc)
- Candidate gene identification and validation
Gene expression:
- Microarray
- RT-PCR
- Quantitative realtime PCR
- In situ hybridization
- Western blot
- Immunohistochemistry